TNFR1 Antibody

• Catalog Number: orb621207
• Category: Antibodies
• Description: TNFR1 Antibody
• Species/Host: Mouse
• Clonality: Polyclonal
• Clone Number: 2G2
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Amino acids 30-301 mapping within the extracellular domain of TNF-R1 of human origin
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:100-1:1,000, IHC: 1:50-1:500, IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
• Form/Appearance: Liquid
• Purity: Affinity purified
• Conjugation: Unconjugated
• MW: Calculated MW 55 kDa
• UniProt ID: P19438
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1*TBS (pH7.4), 1%rAlbumin, 40%Glycerol. Preservative: 0.05% Sodium Azide
• Background: Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2 are expressed on most cell types. The majority of TNF functions are primarily mediated through TNF-R1, while signaling through TNF-R2 occurs less extensively and is confined to cells of the immune system. Both of these proteins belong to the growing TNF and nerve growth factor (NGF) receptor superfamily, which includes FAS, CD30, CD27 and CD40. The members of this superfamily are type I membrane proteins that share sequence homology confined to the extracellular region. TNF-R1 shares a motif coined the death domain with FAS and three structurally unrelated signaling proteins, TRADD, FADD and RIP. This death domain is required for transduction of the apoptotic signal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TNF-R1 expression in MCF7 (A), HeLa (B) and U-937 (C) whole cell lysates

Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells