TNFRSF1A Rabbit Polyclonal Antibody

SKU: orb592651

Description

Rabbit polyclonal antibody to TNFRSF1A

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human
• Predicted Reactivity: Guinea pig, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TNFRSF1A
• Target: TNFRSF1A
• Molecular Weight: 50 kDa
• Purification: Affinity Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

FPF, p55, p60, TBP1, TNF-R, TNFAR, TNFR1, p55-R, CD120a, TNFR55, TNFR60, TNF-R-I, TNF-R55

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. The protein is processed to ~48 kDa, a smaller isoform of 24 kDa also contains the peptide sequence, and the protein may also be glycosylated.

Sample Tissue: Human A549 Whole Cell, Antibody Dilution: 1 ug/ml.

Sample Tissue: Human Jurkat Whole Cell, Antibody Dilution: 1 ug/ml.

Positive control (+): THP-1 (N30), Negative control (-): SH-SY5Y (N19), Antibody concentration: 1 ug/ml.

Immunofluorescent TNFRSF1A detection in human lymphocytes (green fluorescence). Nuclei were stained with DAPI (blue fluorescence), Working dilution 5-10 ug/ml.

WB Suggested Anti-TNFRSF1A Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: DU145 cell lysate. TNFRSF1A is supported by BioGPS gene expression data to be expressed in DU145.

Quick Database Links

Gene Symbol

TNFRSF1A

UniProt

P19438

RefSeq (Protein):NP_001056