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TNF beta Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The ED50 was determined by the cytolysis of murine L929 cells in the presence of Actinomycin - D is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg. |
| Target | LTA |
| Protein Sequence | MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Storage & Handling
−| Storage | The lyophilized TNF beta recombinant protein is stable for at least 2 years from date of receipt at -20˚°C. Reconstituted TNF beta is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚°C. As with any protein, exposing TNF beta recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Disclaimer | For research use only |
Alternative Names
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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 22.79 KDa. Observed: 29-40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman LTBR [orb2993989]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 48.8 KDa. Observed: 61 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgMouse PTX3 [orb2993117]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 43.4 KDa. Observed: 54 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgRecombinant Human Tumor necrosis factor receptor superfamily member 3 (LTBR), partial [orb2658708]
Greater than 90% as determined by SDS-PAGE.
25.8 kDa
E.coli
1 mg, 100 μg, 20 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000586.2 |
|---|
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Protocol Information
TNF beta Recombinant Protein (orb1230833)
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