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Catalog Number | orb1230176 |
---|---|
Category | Proteins |
Description | TNF-alpha multimeric Recombinant Protein |
Tag | DDDDK Tag |
Concentration | 0.1mg/ml after reconstitution. |
Form/Appearance | Lyophilized |
Purity | > 95% (SDS-PAGE). Endotoxin level is less than 0.02EU/ μg purified protein (LAL test; Lonza). |
Target | TNF |
UniProt ID | P01584 |
Protein Sequence | The extracellular domain of human TNF-alpha (aa 85-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a DDDDK-tag. |
Source | HEK293 cells |
Biological Activity | Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells). |
NCBI | P01375 |
Storage | Stable for at least 6 months after receipt when stored at -20˚°C. |
Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
Alternative names | ACRP30headless:TNF-alpha, ACRP30headless:TNFSF2, A Read more... |
Note | For research use only |
Application notes | This recombinant proteins is for research use only. |
Expiration Date | 6 months from date of receipt. |