TMPD dihydrochloride

SKU: orb1940269

Description

TMPD dihydrochloride is an active substrate of enzymatically convert redox and an electron donor for the reduction of heme peroxidases.(In Vitro):TMPD dihydrochloride differentiates organisms that exhibit CYP C oxidase activity and distinguishes between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria. TMPD dihydrochloride can be used as a colorimetric indicator.

Key Properties

• CAS Number: 637-01-4
• MW: 237.17
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₈Cl₂N₂
• SMILES: CN(C)C1=CC=C(N(C)C)C=C1.Cl.Cl
• Solubility: In Vitro: H2O : 100 mg/mL (421.64 mM)

Bioactivity

• In Vitro:
  TMPD (N, N, N′, N′-tetramethyl-para-phenylene-diamine) is used in cell culture and microbiology to differentiate organisms that exhibit cytochrome c oxidase activity and to distinguish between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria. The concept of TMPD oxidation by microorganisms is widely used and is known as a successful colorimetric indicator for bacterial oxidases, as the radical cation TMPD+ ̇, formed by oxidation, shows a characteristic deep blue colour. The electrochemical recognition of the TMPD oxidation by bacterial oxidases can be applied to a variety of pathogens.
  Read more...

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only