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| Catalog Number | orb573647 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to TLR2 |
| Target | TLR2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Goat, Guinea pig, Mouse, Rat, Sheep, Zebrafish |
| Form/Appearance | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human TLR2 |
| Protein Sequence | Synthetic peptide located within the following region: LEPIEKKAIPQRFCKLRKIMNTKTYLEWPMDEAQREGFWVNLRAAIKS |
| UniProt ID | O60603 |
| MW | 90 kDa |
| Tested applications | IF, IHC-P, WB |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | TIL4, CD282 |
| Note | For research use only |
| NCBI | NP_003255 |
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B. Sayyaf Dezfuli a, F. Pironi a, G. Castaldelli b, L. Giari b, M. Lanzoni b, K. Buchmann c, P.W. Kania c, G. Bosi Anguilla anguilla vs Contracaecum rudolphii: Granuloma allows host tolerance and parasite survival Aquaculture, (2024)
Applications
IHC
Sample Type: Human Macrophange Cells, Green: primary, Red: phallodin, Blue: DAPI, Yellow: green/red, Primary dilution: 1:200, Secondary Antibody: anti-Rabbit IgG-FITC, Secondary dilution: 1:1000.
25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 0.05 ug/ml of the antibody was used in this experiment. An isoform containing the peptide sequence is present at 23 kDa.
Sample Tissue: Human Lung Tumor, Antibody dilution: 1 ug/ml.
Sample Tissue: Human OVCAR-3, Antibody dilution: 1.0 ug/ml.
Sample Type: 786-0 Whole Cell lysates, Antibody dilution: 0.05 ug/ml.
IF Information: human macrophages.
IHC Information: Lung.
Immunohistochemistry with Liver tissue at an antibody concentration of 5.0 ug/ml using anti-TLR2 antibody (orb573647).
Immunohistochemistry with pFA fixed human thymus tissue.
TLR2 antibody - C-terminal region (orb573647) validated by WB using Fetal Brain Lysate at 1 ug/ml.
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TLR2 Rabbit Polyclonal Antibody [orb11487]
IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Gallus, Mouse, Porcine, Sheep
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 200 μl, 50 μl - Item 1 of 6
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