TLR2 Rabbit Polyclonal Antibody

• Catalog Number: orb186268
• Category: Antibodies
• Description: TLR2 Rabbit Polyclonal Antibody
• Target: TLR2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human TLR2 (301-400/784aa)
• UniProt ID: O60603
• MW: 84 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Toll-like receptor 2; Toll like receptor 2; Toll l
• Research Area: Defensins, Immune System, Inflammation, Inflammato
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wu, H. et al. Activation of TLR signalling regulates microwave radiation-mediated impairment of spermatogenesis in rat testis Andrologia, 50, (2018)

PubMed: 28782295

Reactivity

Rat

Sample: Lane 1: Spleen (Rat) Lysate at 40 ug, Lane 2: Bronchus (Rat) Lysate at 40 ug, Lane 3: Lung (Rat) Lysate at 40 ug, Primary: Anti-TLR2 (orb186268) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kD, Observed band size: 98 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TLR2 Polyclonal Antibody, Unconjugated (orb186268) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TLR2 Polyclonal Antibody, Unconjugated (orb186268) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.