Tizoxanide

SKU: orb1225933

Description

Tizoxanide is the main metabolite of nitazoxanide, which is a synthetic nitrothiazolyl-salicylamide derivative and an antiprotozoal agent.Parasite Infection Approved(In Vitro):Tizoxanide (TIZ) induces mild mitochondrial uncoupling and activate AMPK in hepatocytes.Tizoxanide (TIZ) inhibits hepatitis C virus (HCV) replication in HCV replicon systems.(In Vivo):Tizoxanide (TIZ) (10 mg/kg; i.v.) can pass the BBB and distribute to the brain tissue. Tizoxanide retains in brain is obviously longer than that in plasma.

Key Properties

• CAS Number: 173903-47-4
• MW: 265.2453
• Purity: >98% (HPLC)
• Formula: C₁₀H₇N₃O₄S
• SMILES: O=C(NC1=NC=C([N+]([O-])=O)S1)C2=CC=CC=C2O
• Target: Antibacterial
• Solubility: DMSO: ≥ 38 mg/mL

Bioactivity

• In Vivo:
  Tizoxanide (TIZ)(10 mg/kg; i. v.) can pass the BBB and distribute to the brain tissue. Tizoxanide retains in brain is obviously longer than that in plasma. Animal model: Sprague Dawley rats. Dosage: 10 mg/kg. Administration: Tizoxanide (10 mg/kg; i. v.). Result: Could pass the BBB and distribute to the brain tissue.
• In Vitro:
  Tizoxanide (TIZ)induces mild mitochondrial uncoupling and activate AMPK in hepatocytes. Tizoxanide (TIZ)inhibits hepatitis C virus (HCV) replication in HCV replicon systems. Cell Viability Assay Cell line: HepG2 cells. Concentration: 1-25 μmol/L. Incubation time: 24 h. Result: Induced mitochondrial uncoupling and AMPK activation not due to the non-specific cytotoxicity in HepG2 cells at concentrations less than 25 μmol/L. Immunofluorescence Cell line: HepG2 cells. Concentration: 10 μmol/L. Incubation time: 24 h. Result: Promoted the nuclear translocation of TFEB by activating AMPK in HepG2 cells. RT-PCR Cell line: HepG2 cells. Concentration: 1-25 μmol/L. Incubation time: 24 h. Result: Induced the increase transcription of SQSTM1/P62 in HepG2 cells. Western blot analysis. Cell line: HepG2 cells. Concentration: 1-25 μmol/L. Incubation time: 24 h. Result: Activated AMPK and increased phosphorylation of ACC in HepG2 cells. Cell Cytotoxicity Assay Cell line: RP7 cells, NTZ-11 and TIZ-9 cell lines. Concentration: The initial drug concentration was 0.02 μM, followed by 0.05, 0.1, 0.5, and 1 μM, and subsequent weekly increases of 1 μM until a final concentration of 11 μM. Incubation time: 3 days. Result: Againsted HCV replication in RP7 cells (IC50: 0.16), NTZ-11 (IC50: 1.2) and TIZ-9 cell lines (IC50: 1.5)

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC697856 | Tizoxanide | Desacetylnitazoxanide | Desacetyl-nitazoxanide

Quick Database Links

Gene Symbol

Antibacterial