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Description
Research Area
Cancer Biology
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | TIMP1 activity was measured by its ability to inhibit human MMP - 1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP - 1 concentration of approximately 0.5 μg/mL, when using an MMP - 1 concentration of 1.6 μg/mL. |
| Target | TIMP1 |
| Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA |
Storage & Handling
−| Storage | The lyophilized TIMP-1 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TIMP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing TIMP-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EPA, EPO, HCI, CLGI, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003245.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide






