Thiazolyl blue tetrazolium bromide

SKU: orb1940950

Description

Thiazolyl blue tetrazolium bromide(MTT).(In Vitro):Preparation of MTT working solution.Dissolve MTT by PBS to obtain 5 mg/mL of MTT.Cell proliferation detection ( 96-well plates).Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h. Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.Add 100 μL DMSO, shock 10 min to dissolve the crystal completely.Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

Key Properties

• CAS Number: 2348-71-2
• MW: 414.32
• Purity: >98% (HPLC)
• Formula: C₁₈H₁₆BrN₅S

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only