Thermolabile UNG (Uracil N-Glycosylase) - 10 u/µl

SKU: orb1821422

Description

Thermolabile UNG (UDG) is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. The enzyme is fully active at temperatures up to 55°C and inactivated above 65°C. Thermolabile Uracyl N-Glycosylase (UNG, UDG) catalyzes the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104to 106copies of U-containing DNA fragments in 2 min at 50°C.

Key Properties

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Storage & Handling

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Storage	store at -20 °C. avoid freeze/thaw cycles
Form/Appearance	liquid
Concentration	10 unit/µl
Disclaimer	For research use only

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