Theophylline monohydrate

SKU: orb1224535

Description

A methylxanthine derivative from tea with diuretic, smooth muscle relaxant, bronchial dilation, cardiac and central nervous system stimulant activities. Mechanistically, theophylline acts as a phosphodiesterase inhibitor, adenosine receptor blocker, and histone deacetylase activator. Theophylline is marketed under several brand names such as Uniphyl and Theochron, and it is indicated mainly for asthma, bronchospasm, and COPD.(In Vitro):Theophylline (1,3-Dimethylxanthine) (1-1000 μM) inhibits cAMP hydrolysis by PDE in homogenates of bronchial tissue to relax human bronchus and pulmonary arteries.Theophylline (1,3-Dimethylxanthine) (10 μg/mL; 24 h; eosinophils) induces apoptosis through a reduction in the antiapoptotic protein Bcl-2.Theophylline (1,3-Dimethylxanthine) (0-500 μM; 2 h; A549 cells) inhibits NF-κB activation, I kappa B alpha (I-κBα) degradation and decreases the level of IL-6 in a concentration-dependent manner.Theophylline (1,3-Dimethylxanthine) (0-1000 μM; 30 min; A549 cells) induces histone deacetylase activity to decrease inflammatory gene expression.(In Vivo):Theophylline (1,3-Dimethylxanthine) (100 mg/kg; i.p.; daily, for 9 d; male Swiss mice) has anti-inflammatory activity in mice and increases IL-6 and IL-10 levels and inhibits TNF-α and NO.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 5967-84-0
• MW: 198.18
• Purity: >98% (HPLC)
• Formula: C₇H₈N₄O₂·H₂O
• SMILES: O.CN1C2=C(NC=N2)C(=O)N(C)C1=O
• Target: AChR
• Solubility: Soluble in Water

Bioactivity

• In Vivo:
  Theophylline (1, 3-Dimethylxanthine) (100 mg/kg; i.p.; daily, for 9 d; male Swiss mice) has anti-inflammatory activity in mice and increases IL-6 and IL-10 levels and inhibits TNF-α and NO. Animal model: Male Swiss mice. Dosage: 100 mg/kg. Administration: Intraperitoneal injection; daily, for 9 days. Result: Increased IL-6 and IL-10 levels and inhibited TNF-α and NO.
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• In Vitro:
  Theophylline (1, 3-Dimethylxanthine) monohydrate (1-1000 μM) inhibits cAMP hydrolysis by PDE in homogenates of bronchial tissue to relax human bronchus and pulmonary arteries. Theophylline (1, 3-Dimethylxanthine) monohydrate (10 μg/mL; 24 h; eosinophils) induces apoptosis through a reduction in the antiapoptotic protein Bcl-2.Theophylline (1, 3-Dimethylxanthine) monohydrate (0-500 μM; 2 h; A549 cells) inhibits NF-κB activation, I kappa B alpha (I-κBα) degradation and decreases the level of IL-6 in a concentration-dependent manner. Theophylline (1, 3-Dimethylxanthine) monohydrate (0-1000 μM; 30 min; A549 cells) induces histone deacetylase activity to decrease inflammatory gene expression. Western blot analysis. Cell line: A549 cells. Concentration: 0, 20, 100 and 500 μM. Incubation time: 2 hours. Result: Decreased the expression of NF-κB p65 and I-κBα degradation in a concentration-dependent manner. Western blot analysis. Cell line: Eosinophils. Concentration: 10 μg/mL. Incubation time: 24 hours. Result: Decreased the expression of Bcl-2.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

AChR