Theaflavin

SKU: orb1299669

Description

Theaflavin, a polyphenolic flavonoid from black tea, exhibits antioxidant, anticancer, anti-inflammatory, and antiviral activities. It is widely used in biochemical research for in vitro and in vivo studies exploring its mechanisms in oxidative stress, inflammation, and oncology.

Research Area

Infectious Disease & Virology, Metabolism Research

Key Properties

• CAS Number: 4670-05-7
• MW: 564.49
• Purity: 99.86% (May vary between batches)
• Formula: C₂₉H₂₄O₁₂
• SMILES: O[C@@H]1Cc2c(O)cc(O)cc2O[C@@H]1c1cc(O)c(O)c2c1cc(cc(O)c2=O)[C@H]1Oc2cc(O)cc(O)c2C[C@H]1O
• Target: Endogenous Metabolite,Influenza Virus
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (1.77 mM);DMSO:42.5 mg/mL (75.29 mM)

Bioactivity

• In Vivo:
  In the pretreated ICH rats Theaflavin significantly alleviated the behavioral defects, protected BBB integrity, and decreased the formation of cerebral edema and the levels of ROS as well as inflammatory cytokines (including interleukin-1 beta [IL-1β], IL-18, tumor nectosis factor-alpha, interferon-γ, transforming growth factor beta, and (C-X-C motif) ligand 1 [CXCL1]).Nissl staining and TUNEL displayed Theaflavin could protect against the neuron loss and apoptosis via inhibiting the activation of nuclear transcription factor kappa-β-p65 (NF-κβ-p65), caspase-1, and IL-1β.
• In Vitro:
  Theaflavin inhibits the growth of OVCAR-3 and A2780/CP70 human ovarian cancer cell lines, but not the ovarian epithelial cell line IOSE 364 (IC50s = 11.9, 38.5, and >40 μM, respectively).
• Cell Research:
  Cell proliferation assay, Hoechst 33342 staining assay, Caspase-Glo Assay, western blot, human umbilical vein endothelial cell tube formation assay and vascular endothelial growth factor (VEGF) enzyme-linked immunosorbent assay were performed.
• Animal Research:
  ICH rat models were induced with type VII collagenase and pretreated with TF by gavage in different doses (25 mg/kg-100 mg/kg). Twenty-four hours after ICH attack, evaluated the rats' behavioral performance, the blood-brain barrier (BBB) integrity, and the formation of cerebral edema. The levels of reactive oxygen species (ROS) and inflammatory cytokines were examined by 2',7'-dichlorofluorescin diacetate and enzyme-linked immunosorbent assay. Nissl staining and transferase dUTP nick end labeling (TUNEL) were aimed to detect the neuron loss and apoptosis, the mechanism of which was explored by Western blot.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Theaflavin, Inhibitor, EndogenousMetabolite, Endogenous Metabolite, InfluenzaVirus, Influenza Virus, Influenza A virus, inhibit

Compound Identifiers

PubChem CID

114777