TH5427

SKU: orb1303484

Description

TH5427 is a potent and selective small molecule inhibitor of NUDT5, exhibiting an IC50 of 29 nM in MG assays. This compound is a valuable chemical probe for studying NUDT5's role in ADP-ribose metabolism and related signaling pathways in cellular and biochemical research contexts.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 2253744-56-6
• MW: 491.33
• Purity: 99.54% (May vary between batches)
• Formula: C₂₀H₂₀Cl₂N₈O₃
• SMILES: C(N1C2=C(N=C1N3CCNCC3)N(C)C(=O)N(C)C2=O)C=4OC(=NN4)C5=CC(Cl)=C(Cl)C=C5
• Target: NUDIX hydrolase,Others,DNA/RNA Synthesis
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:3.67 mg/mL (7.47 mM)

Bioactivity

• Target IC50:
  NUDT5:29 nM(MG assay)
• In Vitro:
  In HL-60 cell lysates, TH5427(20 μM) stabilized NUDT5 to nearly the same levels as observed in the 37 °C DMSO control. TH5427 was the most potent stabilizer under the described conditions with an apparent EC50 of 2.1 μM. Accordingly, TH5427 had a positive, dose-dependent shift in the apparent Tagg—the temperature at which 50 % of the target protein denatures and aggregates (TH5427 shifted NUDT5 Tagg up to ≈ 8 °C).
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

breast cancer, ADP-ribose, ATP synthesis, NUDIX hydrolase, NUDIXhydrolase, NUDT5, inhibit, Inhibitor, TH5427, TH-5427, TH 5427, progestin-dependent, proliferation

Compound Identifiers

PubChem CID

132472992