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Catalog Number | orb1227865 |
---|---|
Category | Proteins |
Description | TGF Beta 1 Recombinant Protein |
Tested applications | WB |
Tag | Tag free |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
MW | 12.8 kDa (monomer) |
Target | Tgfb1 |
UniProt ID | P01137 |
Protein Sequence | Ala 279 - Ser 390 |
Source | HEK293 cells |
Biological Activity | The bio-activity was determined by its ability to inhibit IL-4 induced HT-2 cell proliferation. The ED502X107 Unit/mg |
NCBI | P01137 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | TFA and acetonitrile |
Alternative names | TGFB1, CED, DPD1, LAP, TGFβ1 , TGF-beta Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human TGFB1, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
ELISA, FC | |
Human, Monkey, Mouse | |
Monoclonal | |
Unconjugated |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
43.1 kDa | |
HEK293 cells |
Greater than 85% as determined by SDS-PAGE. | |
30.7 kDa | |
Mammalian cell |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
ICC, WB | |
Human, Mouse | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |