TGF beta 1 Antibody

SKU: orb345420

Description

TGF beta 1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000 - 1:20,000, WB: 1:100 - 1:500
• Reactivity: Human
• Application Notes:
  Anti-TGF beta 1 Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 12.5 kDa in size corresponding to reduced mature TGFb1 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: TGF beta 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length).
• Purity: Rabbit Anti-TGF beta 1 affinity purified antibody is directed against human TGFb1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGFb1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.19 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-TGFB1 antibody, rabbit anti-TGF beta 1 antibody, rabbit anti-TGF ß 1 antibody, Transforming growth factor beta-1, Latency-associated peptide, LAP, TGFB 1

ELISA results of purified Rabbit anti-TGF Beta 1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).

Expression of TGF-β in the lung parenchyma after CLP. Non-diabetic, diabetic and insulin-treated diabetic rats were subjected to CLP or SHAM (false operated) surgery. After 6 hours, the lungs were washed, removed and processed. The expression of TGF-β was assessed by immunohistochemistry, positive staining in brown (diffused) and nuclei in blue (A) and morphometric analysis (B) of stained area in µm2 at 400x magnification. Ten random non-coincident microscopic fields were evaluated for each group, n = 7/group. Scale bar = 20 µm. Data are presented as mean + -SEM. *p < 0.01.

Western blot analysis using Biorbyt's Affinity Purified anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG (H&L) diluted 1:2500.

Western Blot of Rabbit anti-TGF Beta 1 antibody. Lane 1: Reduced recombinant TGF Beta 1. Lane 2: Ladder. Lane 3: Non-reduced recombinant TGF Beta 1. Load: 250 ng per lane. Primary antibody: TGF Beta 1 antibody at 1:200 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637). Predicted/Observed size: 12 kDa/25 kDa, 12 kDa for reduced TGF Beta 1/25 kDa for non-reduced TGF Beta 1.

Quick Database Links

UniProt

P01137

RefSeq:339558