TEV Protease

SKU: orb1821506

Description

TEV Protease is a highly sequence-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The TEV Protease recognition sequence is Glu-Asn-Leu-Tyr-Phe-Gln-(Ser) [ENLYFQ(S)] and cleavage occurs between the Gln and Ser residues. The amino acid in the P1' position can also be G, A, M, C, or H. TEV Protease is used to cleave affinity tags from fusion proteins. The protease has a His-tag for easy removal from a reaction using nickel affinity resins.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 139946-51-3
• MW: Theoretical MW: 30 kDa
• Purity: > 85% (SDS-PAGE)

Storage & Handling

• Storage: store at -20 °C. avoid freeze/thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tobacco Etch Virus Protease, recombinant, E. coli