Tetrahydrobiopterin

SKU: orb1706549

Description

Tetrahydrobiopterin (BH4) is an essential enzymatic cofactor for nitric oxide synthase (NOS) and aromatic amino acid hydroxylases. It is widely used in research models of endothelial dysfunction, including hypertension and diabetes, for both in vitro enzyme studies and in vivo mechanistic investigations.

Research Area

Immunology & Inflammation, Metabolism Research

Key Properties

• CAS Number: 17528-72-2
• MW: 241.25
• Purity: 99.82% (May vary between batches)
• Formula: C₉H₁₅N₅O₃
• SMILES: CC(O)C(O)C1CNc2[nH]c(N)nc(=O)c2N1
• Target: NO Synthase,Endogenous Metabolite,NOS
• Solubility: DMSO:40 mg/mL (165.8 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.29 mM)

Bioactivity

• In Vivo:
  To assess the levels of tetrahydrobiopterin in the retina, three to five retinal pools were collected from WT and HPH-1 mice at postnatal ages 7, 14, and 22 years and evaluated by LC-MS/MS. LC-MS/MS analysis confirmed that the concentration level of tetrahydrobiopterin in the retinal tissue of HPH-1 mice was significantly reduced by approximately 90% compared with WT groups P7, P14, and P22, respectively.
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• In Vitro:
  Microglia cultures under hyperoxic conditions were supplemented with or without Tetrahydrobiopterin (100 μM). Exposure of microglia to hyperoxia-induced oxidative stress for 24 h showed a significant increase in TSP-1 mRNA expression and protein compared with normoxia (21% O2). Tetrahydrobiopterin supplementation significantly prevents hyperoxia-induced microglial activation and prevents microvascular damage in choroidal explants by reducing Iba-1 and TSP-1 expression.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, (Rac)-Sapropterin, BH4, BH-4, BH 4, EndogenousMetabolite, Human Endogenous Metabolite, NOS, Sapropterin, Tetrahydrobiopterin

Compound Identifiers

PubChem CID

135402045