Tetracycline

SKU: orb1310175

Description

Tetracycline is a broad-spectrum antibiotic used in research to study bacterial infections in vitro and in vivo. It is also investigated for its potential to induce tumor regression through BCR-ABL gene silencing in oncological studies.

Research Area

Infectious Disease & Virology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 60-54-8
• MW: 444.43
• Purity: 98.47% (May vary between batches)
• Formula: C₂₂H₂₄N₂O₈
• SMILES: [H][C@@]12C[C@@]3([H])C(=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@H]2N(C)C)C(=O)c1c(O)cccc1[C@@]3(C)O
• Target: Antibiotic,Antibacterial,ribosome
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:15 mg/mL (33.75 mM);DMSO:262.5 mg/mL (590.64 mM)

Bioactivity

• Target IC50:
  MCF-7 cells:41.6 μM|HaCaT cells:8 μM|MG-63 cells:180 μg/mL|HeLa cells:> 400 μg/mL|CCRF-CEM cells:40.5 μM (CC50)|Osteoblast:180 μg/mL
• In Vivo:
  METHODS: To detect in vivo antitumor activity, Binimetinib (5 mg/kg) and BMK120 (7.5 mg/kg) were administered by gavage to athymic (nu/nu) mice bearing A549 xenografts once daily for 21 days. RESULTS: Binimetinib and BKM120 alone at the doses tested only weakly inhibited the growth of A549 xenografts, but the combination of Binimetinib and BKM120 significantly inhibited the growth of A549 xenografts.
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• In Vitro:
  METHODS: Neuroblastoma cells were treated with Binimetinib (0-2 µM) for 24-120 h. Cell viability was measured by MTT assay. RESULTS: Four cell lines, CHP-212, SK-N-BE, SK-N-AS and SJ-NB-10, were sensitive to Binimetinib, achieving <50% survival after 24-120 h of treatment, while five cell lines were resistant to the drug. METHODS: NSCLC cells A549, H157 and H522 were treated with Binimetinib (0.5-1 µM) for 48 h. Cell cycle was measured by flow cytometry. RESULTS: Binimetinib induced G1 phase block in three sensitive NSCLC cell lines at relatively low concentration ranges, e.g. 0.5 and 1 µM.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

oral, Inhibitor, inhibit, Major prion protein, Antibiotic, Bacterial, broad-spectrum, 30S ribosome, 16S ribosome, Deschlorobiomycin, Tetracyclin, Tetracycline, Tetracyclinum

Compound Identifiers

PubChem CID

54675776