TEPP-46

SKU: orb1299971

Description

TEPP-46

Research Area

Metabolism Research

Key Properties

• CAS Number: 1221186-53-3
• MW: 372.46
• Purity: 99.65%
• Formula: C₁₇H₁₆N₄O₂S₂
• SMILES: Cn1c2cc(sc2c2cnn(Cc3cccc(N)c3)c(=O)c12)S(C)=O
• Target: PKM
• Solubility: DMSO:83.3 mg/mL (223.65 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (13.42 mM)

Bioactivity

• Target IC50:
  A549 cells:> 100 μM|HCT116 cells:89.91 μM|MIAPaCa2 cells:> 75 µM|PKM2:92 nM|BxPC-3 cells:> 75 µM|C6 cells:81.8 μM
• In Vivo:
  METHODS: To investigat the neurotoxicity and its mechanism in mice, TCEP hydrochloride (10-100 mg/kg) was administered by gavage to K nMing mice once a day for 30 days. Results: Compared wit the control group the water intake of high-dose TCEP group was declined significantly, an the organ index of liver and spleen were increased significantly. In addition the escape latency of TCEP exposed mice were longer than that in the control group in water maze test, whil the total swimming course of high-dose TCEP group was elevated an the swimming time in target quadrant was obviously shortened compared wit the control group. TCEP exposure can cause neurotoxicity by increasing thyroid hormones and inducing oxidative damage in mice.
• In Vitro:
  METHODS: Human hepatocellular carcinoma cells, HepG2, were treated with TCEP hydrochloride (100-400 µM) for 3 days, and cell viability was measured by MTT assay. Results the viability of HepG2 was significantly reduced to 25.68% and 70.92% after treatment with 200 and 400 µM TCEP for 3 days, wherea the lowest concentration of TCEP (100 µM) did not significantly reduc the viability of HepG2 (3.44%). METHODS: Human peripheral blood mononuclear cell PBMCs were treated with TCEP hydrochloride (0.5-1 mM for 24 h. Morphological and necrotic Changes were assessed by Hoechst 33342/PI staining. Results: Necrosis was observed in cells treated with 0.5 mM of TCEP.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

inhibit, ML 265, ML265, ML-265, Inhibitor, PKM2, Pyruvate Kinase, TEPP 46, TEPP46, TEPP-46

Compound Identifiers

PubChem CID

44246499