Tempol

SKU: orb1300544

Description

Tempol (Tanol) is a cell-permeable superoxide dismutase mimetic and antioxidant. It is widely used in oxidative stress research, demonstrating neuroprotective, anti-inflammatory, and analgesic properties in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 2226-96-2
• MW: 172.25
• Purity: 99.98%
• Formula: C₉H₁₈NO₂
• SMILES: CC1(C)CC(O)CC(C)(C)N1[O]
• Target: Autophagy,ROS,Reactive Oxygen Species
• Solubility: Ethanol:17.2 mg/mL (99.85 mM);H2O:100 mg/mL (580.55 mM);DMSO:255 mg/mL (1480.41 mM)

Bioactivity

• In Vivo:
  Tempol reduces oxidant stress-mediated renal dysfunction and injury in the rat. Tempol prevents cerebral vessel remodeling in hypertensive rats. Tempol lowers blood pressure and sympathetic nerve activity but not vascular O2- in DOCA-salt rats. Tempol restores mitochondrial and cardiac functions in TNFα-induced oxidative stress and reduces cardiac hypertrophy in chronic hypoxic rats.
• In Vitro:
  Tempol (4-Hydroxy-TEMPO) is a stable, cell-permeable nitroxide that acts as a free radical scavenger and nitric oxide spin trap. Tempol Exhibits radio-protective and anti-proliferative properties. Tempol inhibits oxygen-dependent radiation-induced damage. Tempol induces oxidative stress and enhances the levels of p21WAF1 in HL-60 cells in a dose-dependent manner.
• Cell Research:
  The antiproliferative effect of Tempol is assessed by the MTT assay after 96 h exposure of the cells to the drug. Briefly, 100,000 cells/mL are seeded onto 96-well plates and allowed to grow for 24 h before treatment with different concentrations of Tempol (0.05–1.6 mM).(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ROS, Tanol, Tempol, ReactiveOxygenSpecies, TMPN, Reactive Oxygen Species, 4-Hydroxy-TEMPO, Autophagy, Inhibitor, inhibit

Compound Identifiers

PubChem CID

5395