TEMPOL

SKU: orb1300544

Description

Tempol (Tanol) is a cell-permeable superoxide dismutase mimetic and antioxidant. It is widely used in oxidative stress research, demonstrating neuroprotective, anti-inflammatory, and analgesic properties in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Neuroscience

Key Properties

• CAS Number: 2226-96-2
• MW: 172.24
• Purity: >98%
• Formula: C₉H₁₈NO₂
• SMILES: CC1(C)CC(O)CC(C)(C)N1[O]
• Target: SOD (superoxide dismutase)
• Solubility: Soluble in DMSO (up to 25 mg/ml) or in Ethanol (up to 17 mg/ml)

Bioactivity

• In Vivo:
  Tempol reduces oxidant stress-mediated renal dysfunction and injury in the rat. Tempol prevents cerebral vessel remodeling in hypertensive rats. Tempol lowers blood pressure and sympathetic nerve activity but not vascular O2- in DOCA-salt rats. Tempol restores mitochondrial and cardiac functions in TNFα-induced oxidative stress and reduces cardiac hypertrophy in chronic hypoxic rats.
• In Vitro:
  Tempol (4-Hydroxy-TEMPO) is a stable, cell-permeable nitroxide that acts as a free radical scavenger and nitric oxide spin trap. Tempol Exhibits radio-protective and anti-proliferative properties. Tempol inhibits oxygen-dependent radiation-induced damage. Tempol induces oxidative stress and enhances the levels of p21WAF1 in HL-60 cells in a dose-dependent manner.
• Cell Research:
  The antiproliferative effect of Tempol is assessed by the MTT assay after 96 h exposure of the cells to the drug. Briefly, 100,000 cells/mL are seeded onto 96-well plates and allowed to grow for 24 h before treatment with different concentrations of Tempol (0.05–1.6 mM).(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-Hydroxy TEMPO

Compound Identifiers

PubChem CID

5395