TCS7010

SKU: orb1300485

Description

TCS7010

Research Area

Cell Biology, Epigenetics & Chromatin

Key Properties

• CAS Number: 1158838-45-9
• MW: 588.07
• Purity: 99.69%
• Formula: C₃₁H₃₁ClFN₇O₂
• SMILES: CCN1CCN(CC1)C(=O)Cc1ccc(Nc2ncc(F)c(Nc3ccc(cc3)C(=O)Nc3ccccc3Cl)n2)cc1
• Target: Apoptosis,Aurora Kinase
• Solubility: 10% DMSO+90% Corn Oil:2.5 mg/mL (4.25 mM);DMSO:55 mg/mL (93.53 mM)

Bioactivity

• Target IC50:
  Aurora B:3.4 μM|Aurora A:3.4 nM
• In Vitro:
  TCS7010 is a 2,4-dianilinopyrimidine that selectively and potently inhibits Aurora A. TCS7010 effectively inhibits the proliferation of HCT116 and HT29 cells, with IC50 of 190 nM and 2.9 μM, respectively. The Aurora A selectivity of TCS7010 against Aurora B depends on a single amino acid (Thr217) of Aurora A. In KCL-22 cells, TCS7010 (1-5 μM) increases G2/M cell fraction, induces histone H3 serine 10 phosphorylation, and suppresses mitotic Aurora A autophosphorylation on Thr288. TCS7010 (0.5-5 μM) also suppresses cell proliferation in KCL-22 cells, as well as BCR-ABL-negative leukemia cell lines KG-1 and HL-60. TCS7010 effectively induces apoptosis in KCL-22 cells at 5 μM. In a recent study, TCS7010 is also found to inhibit cell growth of HCT116, HT29, and HeLa cells, with IC50 of 377.6 nM, 5.6 μM, and 416 nM.
• Cell Research:
  Cells are seeded in 384-well plates on day 0 in 50 μL of complete medium and incubated overnight in a 5% CO2 atmosphere at 37 °C. On day 1, 10 μL of Aurora A Inhibitor I is added. On day 4, plates are allowed to reach room temperature, and 30 μL Cell Titer-Glo reagent is added to each well to measure total ATP levels. Plates are read after shaking 15 min at room temperature.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Apoptosis, Aurora A Inhibitor I, Aurora B, Aurora C, Aurora A, AuroraKinase, Aurora Kinase, TCS7010, TCS-7010, TCS 7010

Compound Identifiers

PubChem CID

44139710