TCS 401

SKU: orb1299870

Description

TCS 401 is a potent and selective small molecule inhibitor of protein tyrosine phosphatase 1B (PTP1B), exhibiting a Ki value of 0.29 µM. It serves as a valuable research tool for studying PTP1B's role in insulin and leptin signaling, diabetes, and obesity, with utility in both cellular and biochemical assays.

Research Area

Metabolism Research

Key Properties

• CAS Number: 243966-09-8
• MW: 306.72
• Purity: 98.65% (May vary between batches)
• Formula: C₁₀H₁₁ClN₂O₅S
• SMILES: Cl.OC(=O)C(=O)Nc1sc2CNCCc2c1C(O)=O
• Target: Phosphatase
• Solubility: DMSO:11 mg/mL (35.86 mM)

Bioactivity

• Target IC50:
  PTP1B: 0.29 µM(Ki)
• In Vitro:
  TCS 401 promoted the proliferation and expression of cyclin A and cyclin D1 in RPE cells. TCS 401 induced RPE cells to differentiate toward better contractility and motility. A migration assay proved that inhibiting PTP1B improved the migratory activity of RPE cells. TCS 401 activated extracellular signal-regulated kinase (Erk) and protein kinase B (Akt) phosphorylation. Pretreatment with PD98059 and LY294002 abolished TCS 401-induced activation of Erk, Akt, cell proliferation, and cell migration.
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• Cell Research:
  From the cultured RPE cells treated with TCS 401, cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetracolium bromide assay, and the protein expression levels of cyclin A and cyclin D1 were determined. The effect of TCS 401 on cell differentiation was confirmed by immunostaining for α-smooth muscle actin and by western blot. Cell migration activity and PTP1B signaling mechanism were determined. Migration Assay was used to evaluate cell migration activity. PTP1B signaling mechanism was determined by use of PD98059 and LY294002.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, PTP1B, Phosphatase, TCS401, TCS-401, TCS 401

Compound Identifiers

PubChem CID

56972185