Tau (K18) P301L Mutant Pre-formed Fibrils

• Catalog Number: orb1822356
• Category: Proteins
• Description: Human Recombinant Tau (K18) P301L Mutant Protein Pre-formed Fibrils
• Tested applications: In vitro, In vivo, SDS-PAGE, WB
• Reactivity: Human
• Tag: No tag
• Concentration: 2 mg/ml
• Purity: > 95%
• MW: ~15.1 kDa
• Target: Tau (K18) P301L Mutant
• UniProt ID: P10636
• Protein Sequence: SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
• Protein Length: Partial
• Source: Recombinant
• Expression System: E. coli
• Biological Activity: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.
• Storage: -80°C
• Buffer/Preservatives: 10 mM HEPES, 100 mM NaCl pH 7.4
• Alternative names: Tau PFFs, Tau PFF, Tau protein Pre-formed Fibrils,
• Note: For research use only
• Application notes: Certified > 95% pure using SDS-PAGE analysis. Made with low endotoxin monomer.
• Expiration Date: 6 months from date of receipt.

TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.

TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau Pre-formed Fibrils. Tau Pre-formed Fibrils seed the formation of new tau fibrils when combined with tau monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.

SDS-PAGE of ~15 kDa Active Human Tau Protein K18 P301L Pre-formed Fibrils. Lane 1: MW Ladder. Lane 2: Tau Protein Pre-formed Fibrils.