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Tau (K18) P301L Mutant Monomers
Catalog Number: orb1822358
| Catalog Number | orb1822358 |
|---|---|
| Category | Proteins |
| Description | Human Recombinant Tau (K18) P301L Mutant Protein Monomers |
| Tested applications | In vitro, In vivo, SDS-PAGE, WB |
| Reactivity | Human |
| Tag | No tag |
| Concentration | 2 mg/ml |
| Purity | > 95% |
| MW | ~15.1 kDa |
| Target | Tau (K18) P301L Mutant |
| UniProt ID | P10636 |
| Protein Sequence | SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE |
| Protein Length | Partial |
| Source | Recombinant |
| Expression System | E. coli |
| Biological Activity | Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when tau PFFs are combined with tau monomers. |
| Storage | -80°C |
| Buffer/Preservatives | 10 mM HEPES, 100 mM NaCl pH 7.4 |
| Alternative names | Tau monomer, Tau protein monomer, Tau protein, mic Read more... |
| Note | For research use only |
| Application notes | Certified > 95% pure using SDS-PAGE analysis. Low endotoxin < 5 EU/mL @ 2mg/mL. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE of ~16 kDa Human Tau Protein K18 P301L Monomer. Lane 1: MW Ladder. Lane 2: Tau Protein Monomer
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) in tau K18 P301L monomers over time. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
