Tanshinone I

SKU: orb1304863

Description

Tanshinone I (Tanshinone A) is a bioactive diterpenoid isolated from Salvia miltiorrhiza. It exhibits cytotoxic activity against various cancer cell lines in vitro and has shown anti-tumor effects in animal models, making it a compound of interest for oncology and pharmacology research.

Research Area

Metabolism Research

Key Properties

• CAS Number: 568-73-0
• MW: 276.29
• Purity: 98.70% (May vary between batches)
• Formula: C₁₈H₁₂O₃
• SMILES: Cc1coc-2c1C(=O)C(=O)c1c-2ccc2c(C)cccc12
• Target: Phospholipase
• Solubility: DMSO:2.5 mg/mL (9.05 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  PLA2:11 μM
• In Vivo:
  Tanshinone I demonstrates significant anti-inflammatory effects in rat models of both acute and chronic inflammation, specifically in carrageenan (CGN)-induced paw oedema and adjuvant-induced arthritis (AIA). Employing these classical animal models, Tanshinone I, upon oral administration, significantly mitigates CGN-induced paw oedema with a 47% inhibition at a dosage of 160 mg/kg, in contrast to indomethacin's IC50 of 7.1 mg/kg. Additionally, in the AIA model, it achieves a 27% reduction in secondary inflammation on the 18th day at a daily oral dose of 50 mg/kg, while prednisolone displays a potent 65% inhibition at 5 mg/kg/day.
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• Cell Research:
  RAW 264.7 cells are cultured with DMEM supplemented with 10% FBS and 1% antibiotics under 5% CO2 at 37°C. Briefly, cells are plated in 96-well plates (2×105 cells/well). LPS (1 ug/mL) and Tanshinone I are simultaneously added and incubated for 24 h, unless otherwise specified. The PGE2 concentration in the medium is measured using an EIA kit for PGE2. In order to determine the effects of Tanshinone I on PGE2 production after induction of COX-2, cells are incubated with LPS (1 ug/mL) for 24 h and thoroughly washed. Then, Tanshinone I is added without LPS and the cells are incubated for another 24 h. From the medium, PGE2 concentrations are measured. The cytotoxicity of Tanshinone I to RAW cells is checked using the MTT assay. Tanshinone I does not show any cytotoxicity up to 100 uM.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Tanshinone A, Tanshinone I, Phospholipase, PLA2

Compound Identifiers

PubChem CID

114917