TAI-1

SKU: orb1685627

Description

TAI-1 is a specific small molecule inhibitor that targets Hec1, effectively disrupting its interaction with Nek2. This compound is a valuable research tool for studying mitotic progression and has been utilized in both in vitro and in vivo cancer research models to investigate novel anti-mitotic strategies.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 1334921-03-7
• MW: 431.51
• Purity: 99.58%
• Formula: C₂₄H₂₁N₃O₃S
• SMILES: COc1ccc(Oc2cc(C)c(-c3csc(NC(=O)c4ccncc4)n3)c(C)c2)cc1
• Target: Microtubule Associated,MAPK,Apoptosis
• Solubility: DMSO:80 mg/mL (185.4 mM);10% DMSO+90% Corn Oil:3.3 mg/mL (7.65 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  K562 cells:13.48 nM (GI50)
• In Vivo:
  TAI-1 (20 mg/kg i.v. or 150 mg/kg p.o.) causes significant tumor growth delay in Huh-7 model and modest tumor inhibition in Colo205 and MDA-MB-231 models.
• In Vitro:
  TAI-1 disrupts the binding of Nek2 to Hec1, which leads to degradation of Nek2 and chromosomal misalignment. TAI-1 shows strong growth inhibitory potency at nM levels across a broad spectrum of tumor cells, and produces synergistic activity with doxorubicin, topotecan and paclitaxel in leukemia, breast and liver cancer cells.
• Cell Research:
  Cells are seeded in 96 well plates, incubated for 24 hours, compounds added and incubated for 96 hours. All testing points are tested in triplicate wells. Cell viability is determined by MTS assay using CellTiter 96 Aqueous Non-radioactive Cell Proliferation Assay system according to manufacturer's instructions with MTS and PMS. Data retrieved from spectrophotometer are processed in Excel and GraphPad Prism 5 to calculate the concentration exhibiting 50% growth inhibition (GI50). All data represent the results of triplicate experiments.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, inhibit, Microtubule Associated, MicrotubuleAssociated, Inhibitor, Hec1, TAI 1, TAI1, TAI-1

Compound Identifiers

PubChem CID

53389629