Tacedinaline

SKU: orb1307262

Description

Tacedinaline

Research Area

Cell Biology, Epigenetics & Chromatin, Molecular Biology

Key Properties

• CAS Number: 112522-64-2
• MW: 269.3
• Purity: 99.15%
• Formula: C₁₅H₁₅N₃O₂
• SMILES: N(C(=O)C1=CC=C(NC(C)=O)C=C1)C2=C(N)C=CC=C2
• Target: Apoptosis,HDAC
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.43 mM);DMSO:33.33 mg/mL (123.77 mM)

Bioactivity

• Target IC50:
  HDAC2:0.9 μM|HDAC1:0.9 μM|HDAC3:1.2 μM
• In Vivo:
  CI-994 exhibits antitumor activity, displaying enhanced cytocidal effects in solid tumors. It inhibits cell growth across various cell types, including rat leukemia BCLO cells (IC50=2.5 µM) and LNCaP cells (IC50=7.4 µM). In A-549 and LX-1 cells, CI-994 (<160 mM) increases the number of cells in the G0/G1 phase while decreasing those in the S phase, effectively inhibiting cell growth and inducing apoptosis.
• In Vitro:
  In models of chemotherapy-resistant pancreatic ductal adenocarcinoma in mice and human prostate tumor, CI-994 consistently demonstrated antitumor activity.
• Cell Research:
  LNCaP cell lines are maintained in RPMI 1640 medium containing 10% fetal bovine serum, 1% penicillin and streptomycin, as the complete culture medium. Cells (2×104) are seeded in 24-well plates and incubated in a 5% CO2 incubator at 37 °C for 1 day. Cultures are treated with CI-994, alone and in combination on day 2 and 4. Cells are washed on day 2 and 4 and media are changed. Mitochondrial metabolism is measured as a marker for cell growth by adding 100 μL/well MTT (5 mg/mL in medium) with 2 hours incubation at 37 °C on Day 6. Crystals formed are dissolved in 500 μL of DMSO. The absorbance is determined using a microplate reader at 560 nm. The absorbance data are converted into cell proliferation percentage. Each assay is performed in triplicate. (Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Acetyldinaline, Apoptosis, CI 994, CI994, CI-994, inhibit, N-acetyldinaline, PD 123654, PD123654, PD-123654, Inhibitor, HDAC1, HDAC2, HDAC3, HDAC, Histone deacetylases, Goe 5549, Goe5549, Goe-5549, Tacedinaline

Compound Identifiers

PubChem CID

2746