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Catalog Number | orb1231484 |
---|---|
Category | Proteins |
Description | Syndecan-1 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 50.3 kDa |
Protein Sequence | Gln 23 - Gly 254 |
Source | HEK293 cells |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | SDC1,Syndecan-1,CD138,SYND1,SDC Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.3 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human Syndecan-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Syndecan-1, Fc Tag at 1 ug/ml (100 uL/well) can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.1-3 ng/mL.
Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 ug/ml (100 uL/well) can bind Human Syndecan-1, Fc Tag with a linear range of 1-2 ng/mL.
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
27.5 kDa | |
HEK293 cells |
Greater than 90% as determined by SDS-PAGE. | |
26.5 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
39.9 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
54.5 kDa | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of CD138-hFc-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |