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syndecan 1/CD138 Antibody

SKU: orb1248254

Description

syndecan 1/CD138 Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 37kDa band observed in lysates of cell line HepG2 and of Gastrointensinal cancer and Spinal Cord cancer (calculated MW of 32.5kDa according to NP_002988.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-REQEATPRPRETTQ
Targetsyndecan 1 / CD138
Molecular WeightApprox 37kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SDC1, syndecan 1, CD138, SDC, SYND1, syndecan, CD138 antigen, heparan sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1, syndecan-1

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syndecan 1/CD138 Antibody

orb1248254 (1 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

syndecan 1/CD138 Antibody (orb1248254)

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0.1 mg
$ 890.00
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