Anti-CD138 antibody(DM45); Rabbit mAb

• Catalog Number: orb757371
• Category: Antibodies
• Description: Anti-CD138 antibody(DM45), Rabbit mAb
• Target: CD138
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• UniProt ID: P18827
• Tested applications: ELISA, FC, IHC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM45
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SDC1, Syndecan-1, CD138, SYND1, SDC
• Note: For research use only

Expi 293 cell line transfected with irrelevant protein (A) and human CD138 (B) were surface stained with Rabbit anti-CD138 monoclonal antibody 1 μg/ml (clone: DM45) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry analysis of antigen binding of rabbit anti-human CD138 mAb (orb757371). (A) orb757371 does not bind to Jurkat cells that do not express CD138. (B) A clear peak shift of orb757371 was seen compared to the control when incubated with CD138-expressing MM.1S cells, indicating strong binding of orb757371 to CD138. Antibodies were incubated at 5 μg/mL.

orb757371 at 5 μg/ml staining CD138 in RPMI-8226 MM xenografts in NSG mice by IHC (SKU# orb757371);

orb757371 at 10 μg/ml staining CD138 in human tonsil tissue by IHC (SKU# orb757371).