Syk Inhibitor II dihydrochloride

SKU: orb1302895

Description

Syk Inhibitor II (hydrochloride)

Research Area

Cardiovascular Research, Epigenetics & Chromatin, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 227449-73-2
• MW: 413.22
• Purity: 100.00%
• Formula: C₁₄H₁₇Cl₂F₃N₆O
• SMILES: Cl.Cl.NCCNc1ncc(C(N)=O)c(Nc2cccc(c2)C(F)(F)F)n1
• Target: Syk,BTK,PKC,5-HT Receptor,Tyrosine Kinases
• Solubility: Ethanol:0.3 mg/mL (0.73 mM);DMSO:11 mg/mL (26.62 mM);PBS(pH7.2):10 mg/mL (24.2 mM)

Bioactivity

• Target IC50:
  BTK:15.5 μM|PKCε:5.1 μM|ZAP70:11.2 μM|ITK:22.6 μM|Syk:41 nM|PKCβII:11 μM|5-HT receptor:460 nM
• In Vivo:
  Syk inhibitor II has been shown to inhibit passive cutaneous anaphylaxis reactions in mice (ID50 = 13.2 mg/kg, s.c.).
• In Vitro:
  Syk inhibitor II has been shown to prevent FcεRI-mediated 5-HT release in RBL-2H3 cells in vitro (IC50 = 460 nM).
• Animal Research:
  ICR mice were passively sensitized by subcutaneously injectinganti-dinitrophenyl (DNP)-coupled IgE under the right ear pinna, while lightly anesthetizing with ether. After 24hr, each mouse was challenged by injecting amixture of DNP-conjugated bovine serum albumin and 200 μl of 0.5% Evans blue solution via the tail vein to induce passive cutaneous anaphylaxis. Thirty minutes after the challenge, the mice were sacrificed to take both ears and the amount of dye from the blueing region was measured. Test compounds or vehicle alone as a control were subcutaneously administered to the mice 30 minbefore the antigen challenge. The dye in the tissues was extracted with formamide and colorimetrically determined at 620 nm. A value obtained by subtracting the dye content of the left ear from the dye content of the right ear was used as the amount of dye leaked into the tissues by the PCA reaction.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PKCε, PKCβ2, ZAP70, Syk, Syk Inhibitor II, Syk Inhibitor II (hydrochloride), Syk Inhibitor II Dihydrochloride, Tyrosine Kinases, TyrosineKinases, Btk, Itk

Compound Identifiers

PubChem CID

90664659