Swainsonine

SKU: orb3054152

Description

Swainsonine is an alkaloid isolated from Astragalus, acts as an inhibitor of α-mannosidase, with anti-tumor activity.

Key Properties

• CAS Number: 72741-87-8
• MW: 173.21
• Purity: >98% (HPLC)
• Formula: C₈H₁₅NO₃
• SMILES: OC1CN2CCCC(O)C2C1O
• Solubility: DMSO : 10 mg/mL (57.73 mM; ultraphonic; ) Ethanol : 10 mg/mL (57.73 mM; ultraphonic)

Bioactivity

• In Vivo:
  Animal model: Eighty-seven sixty-day-old male C57BL/6 mice. Dosage: 1 mg/kg (cisplatin; 0.25 mg/kg, i.p. , every other day for 10 days). Administration: i.p.; twice daily for ten days. Result: Showed a significant reduction in ascites volume of 63.5% was observed in mice and a significant reduction in the percentage of cells in the G0/G1, S and G2 phases.
• In Vitro:
  Cell Viability Assay Cell line: U251, LN444 cells. Concentration: 0, 10, 20, 30, 40 μM. Incubation time: 12 h. Result: Inhibited cell viability in a dose-dependent manner, and shows no cytotoxicity in normal brain cells. Cell Cycle Analysis Cell line: U251 cells. Concentration: 30 μM. Incubation time: 12 h. Result: Induced cell cycle arrest at G2/M phase. Apoptosis Analysis Cell line: U251 cells. Concentration: 30 μM. Incubation time: 12 h. Result: Induced apoptosis with the cleaved-Caspase-3 and cleaved-Caspase-9 expression were obviously increased.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only