Sudan III

SKU: orb1301650

Description

Sudan III (Solvent Red 23) is a lysochrome dye used to stain lipids and hydrophobic substances in biological research. It is widely applied in histology for in vitro fat staining and has been used in studies of lipid metabolism and adipogenesis in both in vitro and in vivo experimental models.

Key Properties

• CAS Number: 85-86-9
• MW: 352.39
• Purity: 97.38% (May vary between batches)
• Formula: C₂₂H₁₆N₄O
• SMILES: O=C1C=Cc2ccccc2C1=NNc1ccc(cc1)N=Nc1ccccc1
• Target: Others
• Solubility: DMSO:1.76 mg/mL (4.99 mM)

Bioactivity

• In Vitro:
  Tissue lipid staining 1. Material preparation: 1) Sudan III working solution: Prepare 0.5–1% Sudan III in ethanol or ethanol-acetone mixture (70% ethanol and 30% acetone). 2) Sections: frozen sections or paraffin sections. 3) Distilled water and hematoxylin (optional, as contrast stain). 2. Staining steps: 1) Tissue fixation and preparation 2) Fix tissue with 10% neutral buffered formaldehyde (no dewaxing is required for frozen sections). 3) Prepare sections using a cryostat (recommended thickness 5–10 μm). 4. Staining 1) Immerse sections in Sudan III working solution and stain for 5–15 minutes (time can be adjusted according to lipid content). 2) Wash off excess dye with distilled water. 5. Contrast staining (optional): Nuclei can be stained with hematoxylin, and rinse with water after staining for 1–2 minutes. 6. Sealing: Use a sealing medium containing glycerol to seal the slides. Avoid using a sealing medium containing organic solvents to avoid discoloration. 7. Microscope observation: Use an optical microscope to observe the orange-red staining signal of lipids.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, 111440 Red, Sudan Red III, Sudan III, Solvent Red 23, Tetrazobenzene-beta-naphthol, Tetrazobenzene-b-naphthol, Tetrazobenzene-β-naphthol

Compound Identifiers

PubChem CID

62331