SU-5402

SKU: orb1300276

Description

SU 5402 is a multi-targeted tyrosine kinase inhibitor exhibiting potent activity against VEGFR2 (IC50=20 nM), FGFR1 (IC50=30 nM), and PDGFRβ (IC50=510 nM). It is widely used in biochemical and cellular assays to study angiogenesis, tumor growth, and signaling pathways in both in vitro and in vivo cancer research models.

Research Area

Stem Cell & Developmental Biology

Key Properties

• CAS Number: 215543-92-3
• MW: 296.32
• Purity: >98%
• Formula: C₁₇H₁₆N₂O₃
• SMILES: C(=C1C=2C(NC1=O)=CC=CC2)C3=C(CCC(O)=O)C(C)=CN3
• Target: Kinase
• Solubility: Soluble in DMSO (up to 10 mg/ml).

Bioactivity

• Target IC50:
  VEGFR2:20 nM|EGFR:30 nM|FGFR1:30 nM|PDGFRβ:510 nM
• In Vivo:
  In mice, SU5416 (25 mg/kg, i.p.) inhibits subcutaneous growth of a panel of tumor cell lines by inhibiting the angiogenic process associated with tumor growth.
• In Vitro:
  SU5402 inhibits VEGF-, FGF-, PDGF- dependent cell proliferation with IC50 of 0.05 μM, 2.80 μM, 28.4 μM, respectively. In HUVECs, SU5416 selectively inhibits VEGF-driven mitogenesis in a dose-dependent manner with IC50 of 0.04 μM. In nasopharyngeal epithelial cells, SU5402 attenuates LMP1-mediated aerobic glycolysis, cellular transformation, cell migration, and invasion. In mouse C3H10T1/2 cells, SU 5402 diminishes the effect of FGF23 on cell differentiation.
• Cell Research:
  Tumor cell lines used in the in vitro growth are cultured in media at 37°C in 5–10% CO2. SU5416 is serially diluted in media containing DMSO (<0.5%) and added to cultures of tumor cells 1 day after the initiation of culture. Cell growth is measured after 96 h using the sulforhodamine B method. IC50s are calculated by curve fitting using four-parameter analysis.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PF-02969207

Compound Identifiers

PubChem CID

5307