STIM1 Antibody

SKU: orb345770

Description

Stim1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1: 10,000, IHC: 5ug/ml, WB: 1ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Anti-Stim I antibody is tested for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Stim 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human Stim 1.
• Purity: Anti-Stim I was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with rat, human, bovine, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: None. Stabilizer: 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (rAlbumin); Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.03 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-STIM1 antibody, Stromal interaction molecule 1, STIM-1, STIM 1, GOK

Immunohistochemistry of Rabbit anti-GOK / STIM1 antibody. Tissue: Colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Rabbit anti-GOK / STIM1 antibody. Tissue: Testis. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western Blot of Rabbit anti-STIM1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348667). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lsyate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Marker: Opal Pre-stained ladder. Load: 35 µg per lane. Primary antibody: STIM1 antibody at 1 ug/ml overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 77kDa for STIM1.

Quick Database Links

UniProt

Q13586

RefSeq (Protein):NP_003147.2