Stavudine

SKU: orb1225361

Description

Stavudine is a nucleoside analog that inhibits reverse transcriptase and has in vitro activity against HIV.(In Vitro):Stavudine (d4T) (50 μM) significantly reduces the expression of the NLRP3 inflammasome gene, IL-18 production and Aβ 42-stimulated cellular production of IL-1β in THP-1 derived macrophages.Stavudine (d4T) (50 μM) inhibits the assembly of the NLRP3 inflammasome complex and down-regulates the phagocytosis of Aβ 42 by macrophages.Stavudine (d4T) (10 μM, 7 or 14 days) significantly induced CEM cells apoptosis, especially after 14 days, and increases hydrogen peroxide levels.(In Vivo):Stavudine (d4T) (500 mg/kg, daily liquid, 2 weeks) can rapidly induce fat depletion and mild liver damage at high dose in male RjOrl Swiss mice.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 3056-17-5
• MW: 224.21
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₂N₂O₄
• SMILES: OC[C@@H]1C=C[C@H](N2C(NC(C(C)=C2)=O)=O)O1
• Target: Reverse Transcriptase
• Solubility: Ethanol: 15 mg/mL (66.9 mM); DMSO: 45 mg/mL (200.7 mM)

Bioactivity

• In Vivo:
  Stavudine (d4T) (500 mg/kg, daily liquid, 2 weeks) can rapidly induce fat depletion and mild liver damage at high dose in male RjOrl Swiss mice. Animal model: Male RjOrl Swiss mice weighing 0.028-0.03 kg. Dosage: 500 mg/kg. Administration: Daily liquid; 2 weeks. Result: Reduced fat weight gain by 58%, 5.7 g in the control group and 4.9 g in the d4T treated group. Significantly elevated plasma ALT and LDH levels. Reduced plasma acetoacetic acid and beta-hydroxybutyric acid levels. Reduced liver and muscle mtDNA levels at high dose concentration of 500 mg/kg.
• In Vitro:
  Stavudine (d4T) (50 μM) significantly reduces the expression of the NLRP3 inflammasome gene, IL-18 production and Aβ 42-stimulated cellular production of IL-1β in THP-1 derived macrophages. Stavudine (d4T) (50 μM) inhibits the assembly of the NLRP3 inflammasome complex and down-regulates the phagocytosis of Aβ 42 by macrophages. Stavudine (d4T) (10 μM, 7 or 14 days) significantly induced CEM cells apoptosis, especially after 14 days, and increases hydrogen peroxide levels.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

d4T | NSC 163661

Quick Database Links

Gene Symbol

Reverse Transcriptase