STAT5A Antibody

SKU: orb345725

Description

STAT5 R31-Me2a antibody

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 1:5000, WB: 1:500
• Reactivity: Human
• Application Notes:
  Anti-STAT5 [Asym-dimethyl Arg31] antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~90.6 kDa corresponding to STAT5 protein by Western Blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: STAT5A [Asym-dimethyl Arg31] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT.
• Target: STAT5A
• Purity: Anti-STAT5 R31/Me2a was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for STAT5 R31/Me2a reacts with human STAT5. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT5 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.75 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-STAT5 Asym-dimethyl Arg31 antibody, rabbit anti-STAT5 R31me2a antibody, STAT-5, STAT 5, STAT5A, Signal Transducer and Activator of Transcription 5A antibody, STAT-5A, Transcription factor STAT5A antibody

Dot blot of Rabbit Anti-STAT5 R31-Me2a antibody. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT5 R31-Me2a antibody at 1:1000 for 90 min at RT. (Exposure time: 82 seconds)

Quick Database Links

Gene Symbol

STAT5A

UniProt

P42229