STAT3-IN-12

SKU: orb1687240

Description

STAT3-IN-12 is a potent inhibitor of the IL-6-induced JAK/STAT3 signaling pathway. It demonstrates anti-tumor effects by suppressing proliferation and migration while inducing apoptosis and cell cycle arrest in vitro, supporting research in cancers such as hepatocellular carcinoma and esophageal cancer.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 2980758-31-2
• MW: 454.56
• Purity: 99.19% (May vary between batches)
• Formula: C₂₈H₃₀N₄O₂
• SMILES: N=1C=2C=C3N=C(C=4C=CC=C(OC)C4)N(C3=CC2N(C1C=5C=CC=C(OC)C5)CCC)CCC
• Target: STAT,Apoptosis
• Solubility: DMSO:80 mg/mL (175.99 mM)

Bioactivity

• Target IC50:
  HepG2 cells:4.32 μM|EC109 cells:3.63 μM
• In Vivo:
  Methods: STAT3-IN-12 (20, 40 mg/kg intraperitoneal injection, daily for 24 days) was used to treat HepG2 cell xenograft tumor model mice to study its effect on tumor growth in mice. Results: STAT3-IN-12 was able to inhibit tumor growth in mice without affecting the body weight of the mice.
• In Vitro:
  Methods: HepG2 and EC109 cells were treated with STAT3-IN-12 (0, 1.25, 2.5, 5, 10 μM, 16-72 hours) and subjected to cell viability assays, cell migration assays, and Western blotting. Results: STAT3-IN-12 inhibited the growth of HepG2 and EC109 cells with IC50 values of 4.32 μM and 3.63 μM, respectively. It selectively inhibited STAT3 tyrosine 705 phosphorylation after 16 hours of treatment.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

STAT,Apoptosis