SR18662

SKU: orb1305930

Description

SR18662 is a potent small molecule inhibitor of Krüppel-like factor 5 (KLF5) with an IC50 of 4.4 nM. This compound demonstrates anti-tumor activity by reducing viability and inducing apoptosis in colorectal cancer cell lines in vitro, supporting its use in oncology research.

Research Area

Cell Biology, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 2505001-62-5
• MW: 420.31
• Purity: 96.75% (May vary between batches)
• Formula: C₁₆H₁₉Cl₂N₃O₄S
• SMILES: ClC1=C(Cl)C=C(/C=C/C(NCC(N2CCN(S(=O)(C)=O)CC2)=O)=O)C=C1
• Target: KLF,DNA/RNA Synthesis
• Solubility: DMSO:45 mg/mL (107.06 mM)

Bioactivity

• Target IC50:
  KLF5:4.4 nM
• In Vivo:
  The effect of SR18662 treatment shows a significant dose-dependent inhibition of xenograft growth in mice and exceeds ML264 at equivalent doses. SR18662 is potential for colorectal cancer therapy.
• In Vitro:
  SR18662 significantly reduces growth and proliferation of CRC cells and shows improved efficacy in reducing viability of multiple CRC cell lines. Flow cytometry analysis following SR18662 treatment shows an increase in cells captured in either S or G2/M phases of the cell cycle and a significant increase in the number of apoptotic cells. SR18662 also reduces the expression of cyclins and components of WNT and MAPK signaling pathways.
• Cell Research:
  Cell lines: HT29, HCT116, DLD-1 and SW620 colorectal cancer cell lines. Concentrations: 0.001 μM-20 μM. Incubation Time: 24 h. Method: KLF5 promoter activity assay: DLD-1/pGL4.18hKLF5p cells are seeded in 96 well plate and treated with SR18662 dissolved in DMSO in the range of 0.001 to 20 μM or equivalent volume of DMSO as control for 24 h, the human KLF5 promoter activity is determined with the ONE-Glo luciferase assay system by a SpectramMax M3 plate reader.
• Animal Research:
  Animal Models: 7-week-old male NuJ/Foxn1nu mice with DLD-1 human colorectal cells injected. Dosages: 5 mg/kg, 10 mg/kg, 25 mg/kg. Administration: I.P.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNASynthesis, DNA Synthesis, KLF5, SR 18662, SR18662, SR-18662, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

146674222