SR-FLICA Caspase 9 Assay Kit

SKU: orb1409934

Description

This in vitro assay employs the red fluorescent inhibitor probe SR-LEHD-FMK to label active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.

Images & Validation

• Application Notes:
  1. Prepare samples and controls., 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 μL DMSO., 4. Dilute FLICA 1:5 by adding 200 μL PBS., 5. Add diluted FLICA to each sample at 1:30 – 1:60. For example, to stain at 1:30, add 10 μL to 290 μL of cultured cells. To stain at 1:60, add 5 μL to 295 μL of cultured cells., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buff er and spin cells., 8. If desired, label with additional stains, such as Hoechst, 7-AAD, or an antibody., 9. If desired, fix or embed cells., 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. SR-FLICA excites at 550-580 nm and emits at 590-600 nm.

Key Properties

• Sample Types: Cell culture

Storage & Handling

• Storage: 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only