SR-3677

SKU: orb1296145

Description

SR-3677 is a potent and selective ROCK2 inhibitor with an IC50 of 3 nM. This compound is a valuable research tool for investigating Rho-kinase signaling in cellular assays and has been utilized in preclinical studies exploring fibrosis, hypertension, and neurological disorders.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1072959-67-1
• MW: 408.45
• Purity: 99.91%
• Formula: C₂₂H₂₄N₄O₄
• SMILES: CN(C)CCOc1cc(ccc1NC(=O)C1COc2ccccc2O1)-c1cn[nH]c1
• Target: Autophagy,ROCK
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.9 mM);DMSO:40 mg/mL (97.93 mM)

Bioactivity

• Target IC50:
  ROCK2:3 nM|ROCK1:56 nM
• In Vivo:
  SR-3677 increases ex vivo aqueous humor outflow in porcine eyes and inhibiting myosin light chain phosphorylation. Continuous exposure of 25 μM SR-3677 increases the outflow facility by 60% at 1 h perfusion, increasing to 70–80% for the 2–5 h time points.
• In Vitro:
  SR-3677 has an IC50 of ~3 nM in enzyme and cell-based assays and has an off-target hit rate of 1.4% against 353 kinases, and inhibits only 3 out of 70 non-kinase enzymes and receptors. The IC50 value of SR-3677 for ROCK-I is 56 nM.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Autophagy, Inhibitor, inhibit, ROK, ROCK, ROCK1, ROCK2, Rho-associated kinase, Rho-associated protein kinase, Rho-kinase, SR 3677, SR3677, SR-3677

Compound Identifiers

PubChem CID

25093235