SPTAN1 Rabbit Polyclonal Antibody

SKU: orb584030

Description

Rabbit polyclonal antibody to SPTAN1

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SPTAN1
• Target: SPTAN1
• Molecular Weight: 285 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

DEE5, NEAS, EIEE5, SPTA2

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Amount and Sample Type: 500 ug mouse brain homogenate, Amount of IP Antibody: 6 ug, Primary Antibody: SPTAN1, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:5000, Gene Name: SPTAN1.

Immunohistochemistry with Kidney tissue at an antibody concentration of 5 ug/ml using anti-SPTAN1 antibody (orb584030).

Immunohistochemistry with NT-2 cell line.

WB Suggested Anti-SPTAN1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: Hela cell lysate. SPTAN1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.

Quick Database Links

Gene Symbol

SPTAN1

UniProt

Q13813

RefSeq (Protein):NP_003118