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| Catalog Number | orb584030 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to SPTAN1 |
| Target | SPTAN1 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit, Rat, Zebrafish |
| Form/Appearance | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Purification | Affinity Purified |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human SPTAN1 |
| Protein Sequence | Synthetic peptide located within the following region: MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQ |
| UniProt ID | Q13813 |
| MW | 285 kDa |
| Tested applications | IHC, IP, WB |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | DEE5, NEAS, EIEE5, SPTA2 |
| Research Area | Epigenetics |
| Note | For research use only |
| NCBI | NP_003118 |

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Amount and Sample Type: 500 ug mouse brain homogenate, Amount of IP Antibody: 6 ug, Primary Antibody: SPTAN1, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:5000, Gene Name: SPTAN1.

Immunohistochemistry with Kidney tissue at an antibody concentration of 5 ug/ml using anti-SPTAN1 antibody (orb584030).

Immunohistochemistry with NT-2 cell line.

WB Suggested Anti-SPTAN1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: Hela cell lysate. SPTAN1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.
ELISA, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF | |
Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Cy5.5 |
ICC, IF | |
Bovine, Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
FITC |
ELISA, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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