SPECA Rabbit Polyclonal Antibody (Cy5.5)

• Catalog Number: orb1049214
• Category: Antibodies
• Description: SPECA Rabbit Polyclonal Antibody (Cy5.5)
• Target: SPTAN1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Cy5.5
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human SPECA (551-650/2472aa)
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Fodrin alpha chain; (ALPHA)II-SPECTRIN; alpha II S
• Research Area: Actin Crosslinking, Cell Biology, Neurology, Neuro
• Note: For research use only

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-SPECA antibody (orb1049214), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IGg-Cy5.5. Dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.