Spermidine

SKU: orb1302472

Description

Spermidine is a ubiquitous endogenous polyamine with key roles in cellular metabolism. It is widely used in research on autophagy, aging, and cellular homeostasis, with applications in both in vitro and in vivo models studying inflammation, lipid metabolism, and cell proliferation.

Research Area

Metabolism Research

Key Properties

• CAS Number: 124-20-9
• MW: 145.246
• Purity: 99.97% (May vary between batches)
• Formula: C₇H₁₉N₃
• SMILES: NCCCCNCCCN
• Target: Endogenous Metabolite
• Solubility: DMSO:250 mg/mL (1721.17 mM);H2O:100 mg/mL (688.47 mM);5% DMSO+95% Saline:1.67 mg/mL (11.5 mM)

Bioactivity

• Target IC50:
  HeLa cells:121.3 μM|c-Src:106.4 ± 13.4nM (EC50)
• In Vivo:
  METHODS: To study the effects on diabetes, Spermidine (10 mM) was administered orally in water to non-obese diabetic (NOD) mice (type 1 diabetes mouse model) for thirty weeks. RESULTS: Treatment of NOD mice with Spermidine resulted in a higher incidence of diabetes, although pancreatic insulitis was not altered.Spermidine modulated pancreatic tissue polyamine levels and increased signs of autophagy.Spermidine resulted in an increase in the proportion of pro-inflammatory T-cells in the pancreatic lymph nodes (pLN) of diabetic mice.
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• In Vitro:
  METHODS: Human neuroblastoma cells SH-SY5Y were treated with Spermidine (0.05-10 µM) for 24-48 h. Mitochondrial metabolic activity was measured by MTT assay and ATP production assay. RESULTS: No effect of Spermidine was observed after 24 h. 0.1 and 1 µM Spermidine significantly increased cellular metabolic activity and ATP production after 48 h of treatment. The most effective concentration of Spermidine, 0.1 µM, induced an 8.2% increase in metabolic activity and a 4% increase in ATP production. METHODS: The retinal pigment epithelial cell line ARPE-19 was treated with H2O2 (300 µM) and Spermidine (10 µM) for 24 h. Apoptosis was detected by Flow cytometry. RESULTS: 300 µM H2O2 significantly increased the frequency of annexin V-positive cells to approximately 25%, but Spermidine significantly inhibited this increase. The results suggest that Spermidine attenuates H2O2-mediated oxidative stress-induced apoptosis in ARPE-19 cells.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

membrane, Inhibitor, EndogenousMetabolite, H2O2, Human Endogenous Metabolite, inhibit, N-(3-Aminopropyl)-1,4-diaminobutane, N-(4-Aminobutyl)-1,3-diaminopropane, cell, Endogenous Metabolite, photosystem, Spermidine, stability, PSII

Compound Identifiers

PubChem CID

1102