SP2509

SKU: orb1305743

Description

SP2509 is a potent and selective inhibitor of the histone demethylase LSD1, demonstrating an IC50 of 13 nM. This small molecule is a valuable research tool for investigating epigenetic mechanisms in cancer and other diseases, applicable in both cellular and biochemical assays.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin

Key Properties

• CAS Number: 1423715-09-6
• MW: 437.90
• Purity: >98%
• Formula: C₁₉H₂₀ClN₃O₅S
• SMILES: C\C(=N/NC(=O)c1cccc(c1)S(=O)(=O)N1CCOCC1)c1cc(Cl)ccc1O
• Target: Protein demethylase
• Solubility: Soluble in DMSO (up to at least 25 mg/ml)

Bioactivity

• Target IC50:
  LSD1:13 nM
• In Vivo:
  In OCI-AML3, SP250 inhibited colony formation and induced apoptosis. In AML cells, SP2509 inhibited the interaction of LSD1 with CoREST, increased promoter-specific H3K4Me3, and induced p53, p21 and C/EBPα. In primary AML cells, SP2509 induced cell proliferation.
• In Vitro:
  In OCI-AML3, SP250 inhibited colony formation and induced apoptosis. In AML cells, SP2509 inhibited the interaction of LSD1 with CoREST, increased promoter-specific H3K4Me3, and induced p53, p21 and C/EBPα. In primary AML cells, SP2509 induced cell proliferation.
• Cell Research:
  Cultured AML cells are treated with SP2509 and/or PS for 96 h. At the end of treatment, cells are washed free of the drugs and 500 cells per condition are plated in methylcellulose and incubated at 37 °C. Colony formation is measured 7–10 days after plating.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HCI-2509

Compound Identifiers

PubChem CID

135743577