Sodium Oxamate

SKU: orb1219607

Description

Sodium Oxamate is an LDH inhibitor. Sodium oxamate (SO) induces G2/M cell cycle arrest via downregulation of the CDK1/cyclin B1 pathway and promotes apoptosis through enhancement of mitochondrial ROS generation.It inhibits L(+)-lactate dehydrogenase and derails the entire gluconeogenic pathway.NETosis and lactate accumulation during LPS induced sepsis in mice was inhibited by sodium oxamate, LDH inhibitor.

Key Properties

• CAS Number: 565-73-1
• MW: 111.03
• Purity: >98% (HPLC)
• Formula: C₂H₂NNaO₃
• SMILES: [Na+].NC(=O)C([O-])=O
• Target: Apoptosis
• Solubility: In Vitro: H2O : 12.5 mg/mL (112.58 mM)

Bioactivity

• In Vivo:
  Animal model: Female Balb/c nude mice injected with CNE-1 cells. Dosage: 750 mg/kg. Administration: Intraperitoneal injection; 750 mg/kg; once daily; 3 weeks. Result: Inhibited the tumor growth when compared to either oxamate alone or irradiation alone.
• In Vitro:
  Cell Proliferation Assay Cell line: CNE-1 and CNE-2 cells. Concentration: 10 μM. Incubation time: 24-72 hours. Result: Showed IC50s of 74.6, 32.4 and 17.8 mM and 62.3, 44.5, 31.6 mM at 24, 48 and 72 h in the CNE-1 and CNE-2 cancer cells, respectively. Apoptosis Analysis Cell line: NPC cells. Concentration: 0, 20, 50 and 100 mM. Incubation time: 48 hours. Result: Showed the increasement of early and late apoptotic cells in a dose-dependent manner. Increased the expression of pro-apoptotic Bax and cleaved-caspase-3, while reduced the anti-apoptotic signals of Bcl-2 and pro-caspase-3.Cell Cycle Analysis Cell line: CNE-1 and CNE-2 cells. Concentration: 0, 20, 50 and 100 mM. Incubation time: 24 hours. Result: Showed a dose-dependent increase in the numbers of CNE-1 and CNE-2 cells in the G2/M phase.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SO, oxamate sodium, Aminooxoacetic acid sodium salt, Oxamic acid sodium salt

Quick Database Links

Gene Symbol

Apoptosis