Sodium citrate

SKU: orb2900715

Description

Citric acid trisodium is a natural preservative and food tartness enhancer. Citric acid trisodium induces apoptosis and cell cycle arrest at G2/M phase and S phase. Citric acid trisodium cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid trisodium causes renal toxicity in mice.

Key Properties

• CAS Number: 68-04-2
• MW: 258.07
• Purity: >98% (HPLC)
• Formula: C₆H₅Na₃O₇
• SMILES: C(C(=O)[O-])C(CC(=O)[O-])(C(=O)[O-])O.[Na+].[Na+].[Na+]
• Solubility: In Vitro: H2O : 50 mg/mL (193.75 mM; Ultrasonic)

Bioactivity

• In Vivo:
  Citric acid trisodium (120, 240, and 480 mg/kg; i.p.) significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver. Citric acid trisodium (120, 240, and 480 mg/kg; i.p.) induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes. Citric acid trisodium (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) causes renal toxicity in mice. Animal model: 20 g male Kunming mice. Dosage: 120, 240, 480 mg/kg. Administration: i.p.; weekly for 3 weeks. Result: T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.
• In Vitro:
  Citric acid trisodium (0-12.5 mM; 24 h) shows antiproliferative activity in a dose dependent manner. Citric acid trisodium (12.5 mM; 72 h) induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner. Citric acid trisodium (12.5 mM; 48 h) increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl.. Cell Viability Assay Cell line: HaCaT cells. Concentration: 0, 2.5, 5, 7.5, 10, 12.5 mM Incubation time: 24 h. Result: Inhibited the cell viability in a dose dependent manner. Cell Cycle Analysis Cell line: HaCaT cells. Concentration: 12.5 mM Incubation time: 0, 12, 24, 48, 72 h. Result: Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner. Western blot analysis. Cell line: HaCaT cells. Concentration: 12.5 mM Incubation time: 12, 24, 48 h. Result: Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only