SN-38

SKU: orb1307686

Description

SN-38

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 86639-52-3
• MW: 392.4
• Purity: 99.70%
• Formula: C₂₂H₂₀N₂O₅
• SMILES: C(C)C1=C2C(C=3N(C2)C(=O)C4=C(C3)[C@](CC)(O)C(=O)OC4)=NC=5C1=CC(O)=CC5
• Target: Drug Metabolite,Autophagy,DNA/RNA Synthesis,ADC Cytotoxin,Topoisomerase
• Solubility: DMSO:50 mg/mL (127.42 mM);Ethanol:< 1 mg/mL (insoluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.93 mg/mL (10.02 mM)

Bioactivity

• Target IC50:
  RNA synthesis:1.3 μM|CLM22 organoids:10.67 nM A549 cells proliferation:200.0 ± 14.9 ng/mL|CWH22 organoids:7.54 nM|MCF-7 cells proliferation:16.0 ± 0.7 ng/mL|DNA synthesis:0.077 μM
• In Vivo:
  METHODS: To tes the antitumor activity in vivo SN-38 (2 mg/kg) was administered by Single intraperitoneal injection to C57B-/- mice transplanted with LLC cells in the peritoneal cavity. Results: A Single intraperitoneal injection of SN-38 significantly attenuate the growth of LLC tumors, resulting in a 22.7% reduction in tumor growth. METHODS: To tes the antitumor activity in vivo SN-38 (10 mg/kg in 0.5% carboxymethylcellulose sodium, intraperitoneal injection) and gefitinib (100 mg/kg, subcutaneous injection) were administered to BALB/c nude mice bearing human oral squamous tumors HSC-2 five times per week for three weeks. Results: Only gefitinib was administered to mice with human oral squamous tumors. Results: There was no significant difference in tumor growth Inhibition between gefitinib only and gefitinib plus SN-38 treatment. however, some tumors in the gefitinib-only group showed new growth when tumor measurements were continued after treatment was stopped.
• In Vitro:
  METHODS: Lung cancer cells LLC, A549 and H358 were treated with SN-38 (10-1000 nM) for 48 h. Cell viability was detected using MTT assay. Results: SN-38 started to exhibit effects at 10 nM concentration and induced about 50% cell death at 100 nM. METHODS: Colorectal cancer cells KM12C, KM12SM and KM12L4a were treated with SN-38 (2.5 µg/mL) for 4-48 h. Cell cycle and apoptosis were detected by Flow cytometry. Results: SN-38 induced S-phase and G2-phase block, with KM12L4a cells responding most strongly in a time-dependent manner. apoptosis increased over time in the KM12SM and KM12L4a cell lines, but there was no such change in the KM12C cells.
• Cell Research:
  MTT assay(Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

ADC Payload, ADC Cytotoxin, ADCCytotoxin, Autophagy, DNA synthesis, DNA Synthesis, DNASynthesis, Inhibitor, inhibit, NK012, NK-012, NK 012, Topoisomerase, Topo I, RNA Synthesis, RNASynthesis, SN 38, SN38, SN-38

Compound Identifiers

PubChem CID

104842