SMCC

SKU: orb1298356

Description

SMCC is a heterobifunctional crosslinker that conjugates amines to thiols, enabling protein-protein and protein-antibody conjugation for biochemical research, including in vitro assay development and antibody-drug conjugate (ADC) studies.

Key Properties

• CAS Number: 64987-85-5
• MW: 334.32
• Purity: 99.53% (May vary between batches)
• Formula: C₁₆H₁₈N₂O₆
• SMILES: O=C(ON1C(=O)CCC1=O)C1CCC(CN2C(=O)C=CC2=O)CC1
• Target: ADC Linker
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.98 mM);DMSO:72.14 mg/mL (215.78 mM)

Bioactivity

• In Vitro:
  N-Succinimidyl 4-(N-maleimidomethyl)cycl（SMCC) is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks.
• Cell Research:
  Branched PEI (25 kDa) was dissolved in PBS buffer to yield a concentration of 1 mg/mL. No-weigh Sulfo-SMCC was dissolved in Milli-Q water with a concentration of 10 mg/mL, then a certain of the prepared no-weigh SulfoSMCC solution was added into 2 mL PEI solution to incubate for 30 min at 37 C. After the PEI-SMCC polymer was formed, TAT solution was added into the polymer at the given molar ratio and incubated for 2 h at 4 C. The concentration of unreacted SMCC and unreacted TAT were removed using Zeba Spin Desalting Columns. The polymer PEI-SMCC-TAT was also characterized and analyzed through FTIR to check if each element was added to the PEI backbone.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SMCC, ADC Linker, ADCLinker, ADC Linkers, Antibody-drug conjugates linkers, inhibit, N-Succinimidyl 4-(N-maleimidomethyl)cycl, Inhibitor

Compound Identifiers

PubChem CID

125175